POSTDOCTORAL RESEARCH SCIENTIST IN PHYTOCHROME STRUCTURAL BIOLOGY
Vierstra Lab: Department of Biology
Washington University in St. Louis, USA
Full Description: A Postdoctoral Research Scientist position is available to join a team that is studying the phytochrome family of photoreceptors at the structural and biochemical levels to understand how they transition between their spectrally and conformationally distinct inactive Pr and active Pfr states. These photoreceptors pervade the microbial and plant worlds and control an assortment of processes important for growth, development, reproduction, motility, pigmentation and pathology. In plants, phytochromes are the dominant photoreceptors capable of triggering a number of critical developmental transitions, including seed germination, seedling growth, chloroplast development, shade avoidance, circadian rhythm entrainment, flowering time and senescence. Thus, understanding how phytochromes signal not only has interest at a basic science level and technology development, but also with respect to crop improvement, the ecology of microbial communites, and disease prevention.
The goals of this newly-funded NIH project is to use an array of structural and biochemical approaches to understand: (i) the early conformational events during the pico- to millisecond transitions between Pr and Pfr using both serial femtosecond crystallography with X-ray free-electron laser sources (XFEL) and temperature scanning crystallography; (ii) the structure of a phytochrome dimer as Pr and Pfr using an assortment of X-ray crystallography and cryo-EM approaches to define the conformational changes that distinguish these two states; (iii) how various isoforms of plant phytochromes exploit their physico-chemical differences to enable perception of both light and temperature; and ultimately (iv) how signaling by Pfr is transmitted to downstream signaling partners, be it microbial phytochromes that work in two-component kinase cascades, or plant phytochromes that employ reversible inactivation of a suite of PIF transcriptional repressors. We then want to exploit this knowledge to engineer plants expressing phytochromes with unique signaling properties for agricultural benefit.
Importantly, all of the methodologies are in place to express spectrally-active phytochromes recombinantly and study them by cryo-EM and X-ray crystallography with standard synchrotron souces as well as by XFEL, using facilities both at Washington University and through various collaborators. Of particular importance is our recent ability to generate difraction-quality crystals of a phytochrome that can transition between the inactive and active states while in a crystal lattice, and the first 3D views of entire plant phytochromes as Pr. See papers by Burgie et al. (2015) PNAS; Burgie et al. (2016) Structure; Legris et al. (2016) Science; Fuller et al., (2017) Nat.Methods; Burgie et al. (2017) Sci. Rep.; Burgie et al., (2020) PNAS; Burgie et al., (2021) PNAS; Li et al. (2022) Nature; and soon to be released atomic-resolution view of a plant PhyA dimer for more background on the topic and experimental approaches. The project not only offers exciting science but also the ability to become proficient in modern 3D structure-based, proteomic, and structure-guided engineering approaches.
Washington University in St. Louis is a center of excellence in all aspects of biology with a special emphasis on plant science, and includes modern facilities and instrumentation necessary for the proposed work. In addition, the Washington University Medical School, the Danforth Plant Science Center and companies such as Bayer Crop Sciences and Benson Hill are nearby, making St. Louis an attractive place to do research with an abundance of technical expertise available. St. Louis is a diverse community which boasts an attractive living environment with numerous cultural, sporting and recreational activities close by.
Application Instructions: Email ([email protected]) or send your resume, copies of relevant publications, a cover letter detailing research experience, and a list of three scientists (preferably faculty) that can provide letters of recommendation to:
Dr. Richard D. Vierstra
George & Charmaine Mallinckrodt ProfessorDepartment of Biology, Washington University in St. LouisOne Brookings Drive, St. Louis, MO 63130.
314-935-5058 office. 608-469-6569 cell
Questions about this position and our recent progress can be addressed via email (rd[email protected]) or by phone (608-469-6569, cell). Applications will be accepted until a suitable candidate is hired.
Ph.D in biochemistry, genetics, molecular biology, or related areas. Experiences with various structural techniques and a broad background in biochemistry would be helpful. A competitive salary (commensurate with experience), fringe benefits including health insurance, and travel support to meetings are available. Funding is for at least 2 years with additional years possible. Washington University in St. Louis is an Equal Opportunity/Affirmative Action Employer.
Research in the Washington University Department of Biology addresses a wide range of biological questions, across and between the sub-disciplines of biology: from single molecules to systems, and from steady state equilibria to dynamic remodeling over milliseconds to millions of generations. Intellectually, the department draws its strength from an exceptionally interactive and collaborative faculty who possess a wide range of interests at all levels of biological organization and who utilize many different biological systems and model organisms. Read more at https://wubio.wustl.edu/.